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null (Ed.)The depth of field (DoF) was extended 2.8-fold to achieve rapid crystal screening by retrofitting a custom-designed micro-retarder array (µRA) in the optical beam path of a nonlinear optical microscope. The merits of the proposed strategy for DoF enhancement were assessed in applications of second-harmonic generation imaging of protein crystals. It was found that DoF extension increased the number of crystals detected while simultaneously reducing the number of ` z -slices' required for screening. Experimental measurements of the wavelength-dependence of the extended DoF were in excellent agreement with theoretical predictions. These results provide a simple and broadly applicable approach to increase the throughput of existing nonlinear optical imaging methods for protein crystal screening.more » « less
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Ulcickas, James R. W.; Ding, Changqin; Deng, Fengyuan; Simpson, Garth J. (, Optics Letters)
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Ding, Changqin; Ulcickas, James R.; Deng, Fengyuan; Simpson, Garth; Brown, Thomas G.; Wilson, Tony (, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI)
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Ulcickas, James R.; Ding, Changqin; Deng, Fengyuan; Simpson, Garth J.; Periasamy, Ammasi; So, Peter T.; Xie, Xiaoliang S.; König, Karsten (, Multiphoton Microscopy in the Biomedical Sciences XVIII)
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